Journal: BMC Microbiology

Article Title: Cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (WEEM)

doi: 10.1186/1471-2180-12-79

Figure Lengend Snippet: Scatter plots of 16 cytokine concentrations detected in human blood following ex vivo bacterial exposures. Cytokine concentrations were displayed on a logarithmic scale. The cytokines shown here were detected out of the 30 cytokines in the arrays. The 8 cytokines that were found to be statistically differentially expressed among these samples are highlighted with rectangular boxes. Each mark delineates the average of triplicate exposure samples. Each exposure sample is loaded onto a protein array chip that contains 5 independent measurements per cytokine meaning that fifteen measurements are used to obtain these data.

Article Snippet: These protein arrays allow the simultaneous quantification of 30 biologically relevant cytokines, as determined by Zyomyx, Inc: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p40), IL-12(p40/p70), IL-12(p70), IL-13, IL-15, TNFα, TNFβ, Eotaxin, MCP-1, MCP-3, TRAIL, CD95(sFas), MIG, sICAM-1, IP-10, CD23, TGF-β, GM-CSF, GCSF, IFN-γ.

Techniques: Ex Vivo, Protein Array

Journal: BMC Microbiology

Article Title: Cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (WEEM)

doi: 10.1186/1471-2180-12-79

Figure Lengend Snippet: Concentrations of 8 cytokines in human whole blood after ex vivo exposure to pathogens. The control was a mock-exposed sample. Cytokine concentrations were determined using protein arrays. The bars represent the average of three replicate samples that each contain 5 replicate features per cytokine assay and the lines represent the standard deviation among the three replicates.

Article Snippet: These protein arrays allow the simultaneous quantification of 30 biologically relevant cytokines, as determined by Zyomyx, Inc: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p40), IL-12(p40/p70), IL-12(p70), IL-13, IL-15, TNFα, TNFβ, Eotaxin, MCP-1, MCP-3, TRAIL, CD95(sFas), MIG, sICAM-1, IP-10, CD23, TGF-β, GM-CSF, GCSF, IFN-γ.

Techniques: Ex Vivo, Cytokine Assay, Standard Deviation

Journal: BMC Microbiology

Article Title: Cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (WEEM)

doi: 10.1186/1471-2180-12-79

Figure Lengend Snippet: Clustering result with HOPACH using the average linkage distance between clusters is shown. The eight pathogen-exposed samples are clustered according to the dendrogram on the left and cluster into five groups, 1) Y. pestis (KIM5, NYC, and India), 2) Y. pseudotuberculosis , 3) Y. enterocolitica , 4) B. anthracis (Ames and Sterne), and 5) Control. Sixteen cytokines (Eotaxin, IL-10, IL-12(p40), IL-15, IL-1α, IL-1β, IL-6, IL-8, IP-10, MCP-1, MIG, TNFα, TRAIL, sCD23, sCD95, and sICAM-1) are also reordered based on their correlations according to the dendrogram on the top. Clusters go from root at top to leaf node for each cytokine. Clusters in between are based on their agglomerative . The branch shows the similarity, the short the branch, the more similar. In addition, the eight rightmost proteins form a cluster that may involve inflammation-related cascades initiated by an innate immune response to these pathogen. Colors represent units of log10 [pg/ml], in ten equally spaced intervals increasing from white to dark red. A key showing the specific log10 values for each interval is shown in the figure.

Article Snippet: These protein arrays allow the simultaneous quantification of 30 biologically relevant cytokines, as determined by Zyomyx, Inc: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p40), IL-12(p40/p70), IL-12(p70), IL-13, IL-15, TNFα, TNFβ, Eotaxin, MCP-1, MCP-3, TRAIL, CD95(sFas), MIG, sICAM-1, IP-10, CD23, TGF-β, GM-CSF, GCSF, IFN-γ.

Techniques: